NADK2 Knockout cell line (Hep G2)

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NADK2 Knockout cell line (Hep G2) Order

NADK2 Knockout cell line (Hep G2)
Catalog#
YKO-HT5511
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NADK2 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NADK2 Knockout cell line (Hep G2)
Species
Human
Gene Name
NADK2
Gene ID
133686
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript NADK2-202)
Validation: PCR+Sanger Sequencing
NADK2 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NADK2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NADK2 gene knockout NADK2 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NADK2 Knockout cell line (Hep G2)
NADK2 Knockout cell line (Hep G2)
Catalog#
YKO-HT5511
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Nadk2 Knockout cell line (LLC)

    Nadk2 Knockout cell line (LLC)
      inquiry   More details

    Organism: Mouse
    Gene Name: Nadk2
    Gene ID: 68646
    Size: 1*10^6
    Catalog#: YKO-M215
    YKO-M215(LLC)
  • NADK2 Knockout cell line (HeLa)

    NADK2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60412
    YKO-XN60412(HeLa)
  • NADK2 Knockout cell line (HCT 116)

    NADK2 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60411
    YKO-XN60411(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Nadk2 Knockout cell line (LLC)

    Nadk2 Knockout cell line (LLC)

    Organism: Mouse
    Gene Name: Nadk2
    Gene ID: 68646
    Size: 1*10^6
    Catalog#: YKO-M215
      inquiry   More details
  • NADK2 Knockout cell line (HeLa)

    NADK2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60412
      inquiry   More details
  • NADK2 Knockout cell line (HCT 116)

    NADK2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60411
      inquiry   More details

NADK2 Knockout cell line (Hep G2) Order

NADK2 Knockout cell line (Hep G2)
Catalog#
YKO-HT5511
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NADK2 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NADK2 Knockout cell line (Hep G2)
Species
Human
Gene Name
NADK2
Gene ID
133686
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript NADK2-202)
Validation: PCR+Sanger Sequencing
NADK2 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NADK2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NADK2 gene knockout NADK2 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NADK2 Knockout cell line (Hep G2)
NADK2 Knockout cell line (Hep G2)
Catalog#
YKO-HT5511
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Nadk2 Knockout cell line (LLC)

    Nadk2 Knockout cell line (LLC)
      inquiry   More details

    Organism: Mouse
    Gene Name: Nadk2
    Gene ID: 68646
    Size: 1*10^6
    Catalog#: YKO-M215
    YKO-M215(LLC)
  • NADK2 Knockout cell line (HeLa)

    NADK2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60412
    YKO-XN60412(HeLa)
  • NADK2 Knockout cell line (HCT 116)

    NADK2 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60411
    YKO-XN60411(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Nadk2 Knockout cell line (LLC)

    Nadk2 Knockout cell line (LLC)

    Organism: Mouse
    Gene Name: Nadk2
    Gene ID: 68646
    Size: 1*10^6
    Catalog#: YKO-M215
      inquiry   More details
  • NADK2 Knockout cell line (HeLa)

    NADK2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60412
      inquiry   More details
  • NADK2 Knockout cell line (HCT 116)

    NADK2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NADK2
    Gene ID: 133686
    Size: 1*10^6
    Catalog#: YKO-XN60411
      inquiry   More details

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